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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.096 | 1.987 |
250.00 | 1.602 | 1.493 |
125.00 | 1.147 | 1.038 |
62.50 | 0.795 | 0.686 |
31.25 | 0.485 | 0.376 |
15.63 | 0.313 | 0.204 |
7.82 | 0.236 | 0.127 |
0.00 | 0.109 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 79-93% | 86% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 85-95% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-93% | 87-96% | 97-105% | 95-102% |
EDTA plasma(n=5) | 78-97% | 86-97% | 81-103% | 88-97% |
Heparin plasma(n=5) | 89-104% | 97-106% | 82-91% | 85-96% |
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