This product is a special reagent for Real Time One Step RT-PCR using the probe method. Using this product for Real Time RT-PCR reaction can continuously perform reverse transcription and PCR amplification in the same reaction tube. It is simple to operate and can effectively prevent contamination. Since this reaction system can monitor the amplified products in real time, the detection sensitivity is greatly improved, and the electrophoresis step after PCR reaction is omitted, which is very suitable for the detection of RNA viruses.This product uses high-efficiency reverse transcriptase and high-specificity hot-start Taq DNA polymerase to perform stable and efficient Real Time One Step RT-PCR reactions. For the fluorescent quantitative PCR instrument that uses ROX as the calibration dye, this product is equipped with a separate ROX dye to correct the fluorescent signal error generated between the wells of the quantitative PCR instrument.
产品特点:
1.只需加入RNA,即可在统一PCR管内一连举行逆转录和PCR扩增,操作很是轻盈。
2.对扩增产品举行实时检测,大大提高了检测迅速度,并能有用避免污染。
3.优选的三代逆转录酶配套热启动Taq酶使用以获得最佳的扩增效果,有用降低了非特异性扩增。
4.省略了PCR反应后的电泳办法,很是适合于微量RNA的检测。
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